ldtЛучевая диагностика и терапияDiagnostic radiology and radiotherapy2079-5343Baltic Medical Education Center10.22328/2079-5343-2026-17-2-22-30ldt-1245Research ArticleЛЕКЦИИ И ОБЗОРЫLECTURES AND REVIEWSМетоды редактирования 1H-магнитно-резонансной спектроскопии: лекцияSpectral editing in 1H-magnetic resonance spectroscopy: a lecturehttps://orcid.org/0000-0002-3235-8854АхадовТ. А.AkhadovT. A.

Ахадов Толибджон Абдуллаевич - доктор медицинских наук, профессор, руководитель отдела лучевых методов диагностики 

127549, Москва, ул. Каргопольская ул., д. 10

Tolibdzhon A. Akhadov - Dr. of Sci. (Med.), Professor, Head of the Department of Radiation Diagnostic Methods

127549, Moscow, Kargopolskaya St., 104

akhadov@mail.ruhttps://orcid.org/0000-0002-4627-9874УблинскийМ. В.UblinskyM. V.

Ублинский Максим Вадимович - кандидат биологических наук, ведущий научный сотрудник отдела лучевых методов диагностики

119180, Москва, ул. Большая Полянка, д. 22

Maxim V. Ublinsky - Cand. of Sci. (Med.), Leading Researcher, Department of Radiation Diagnostic Methods

119180, Moscow, st. Bolshaya Polyanka, 22

maxublinsk@mail.ruhttps://orcid.org/0000-0003-1736-6503ЯковлевА. Н.YakovlevA. N.

Яковлев Алексей Николаевич - научный сотрудник 

119180, Москва, ул. Большая Полянка, д. 22

Alexey N. Yakovlev - Researcher

119180, Moscow, st. B. Polyanka, 22

yakovlevalekcej@bk.ruhttps://orcid.org/0000-0002-4709-9461БожкоО. В.BozhkoO. V.

Божко Ольга Васильевна - кандидат медицинских наук, старший научный сотрудник отдела лучевых методов диагностики 

119180, Москва, ул. Большая Полянка, д. 22

Olga V. Bozhko - Cand. of Sci. (Med.), Senior Researcher, Department of Radiation Diagnostic Methods

119180, Moscow, st. B. Polyanka, 22

bozhko_olga@mail.ruНаучно-исследовательский институт неотложной детской хирургии и травматологии Клиника доктора Рошаля; Московский государственный университет имени М. В. ЛомоносоваРоссияClinical and Research Institute of Emergency Pediatric Surgery and Traumatology — Dr. Roshal’s Clinic; Lomonosov Moscow State UniversityRussian FederationНаучно-исследовательский институт неотложной детской хирургии и травматологии Клиника доктора Рошаля; Институт биохимической физики имени Н. М. Эмануэля РАНРоссияClinical and Research Institute of Emergency Pediatric Surgery and Traumatology — Dr. Roshal’s Clinic; N. M. Emanuel Institute of Biochemical Physics, Russian Academy of SciencesRussian FederationНаучно-исследовательский институт неотложной детской хирургии и травматологии Клиника доктора РошаляРоссияClinical and Research Institute of Emergency Pediatric Surgery and Traumatology — Dr. Roshal’s ClinicRussian Federation2026240720261722230Copyright © Ахадов Т.А., Ублинский М.В., Яковлев А.Н., Божко О.В., 20262026Ахадов Т.А., Ублинский М.В., Яковлев А.Н., Божко О.В.Akhadov T.A., Ublinsky M.V., Yakovlev A.N., Bozhko O.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://radiag.bmoc-spb.ru/jour/article/view/1245ВВЕДЕНИЕ

ВВЕДЕНИЕ: Метод протонной магнитно-резонансной спектроскопии (МР-спектроскопия, МРС) позволяет определять концентрации метаболитов тканей в области интереса. Используемые в клинике импульсные последовательности МР-спектроскопии решают две главные проблемы: локализации вокселя и подавления сигнала воды. Полученные таким образом спектры являются наложением всех сигналов из области интереса, поэтому наиболее достоверно можно получить информацию только о небольшом количестве метаболитов, имеющих наибольшие концентрации. Остальные метаболиты имеют низкую концентрацию и/или имеют сложное расщепление пиков и при этом перекрыты более интенсивными сигналами. Метод выявления этих метаболитов в клинически доступных системах (<3T) — спектральное редактирование. В данной лекции кратко представлены методы спектрального редактирования, рассмотрены методы постобработки таких спектров и основные результаты исследований с применением спектрального редактирования. Методами спектрального редактирования показано, что концентрации γ-аминомасляной кислоты, лактата, аспартата, N-ацетиласпартата и N-ацетиласпартилглутамата, 2-гидроксиглутарата, глутатиона, глутамата изменяются при различных заболеваниях. Рассмотренные в данной лекции методы и результаты исследований позволят клиницистам предположить и апробировать новые подходы к диагностике заболеваний центральной нервной системы.

ЦЕЛЬ

ЦЕЛЬ: Описать методы редактирования спектра 1H-МРС в исследованиях на людях и представить некоторые основные результаты применения этих измерений.

ЗАКЛЮЧЕНИЕ

ЗАКЛЮЧЕНИЕ: Использование наиболее эффективных методов редактирования, оптимизирующих информацию в протонных спектрах головного мозга человека,— технически крайне сложный процесс. Однако применение упоминаемых в лекции методик редактирования позволяет получить уникальную и неоценимую информацию о внутриклеточных концентрациях ряда важнейших метаболитов, что, безусловно, повышает уровень понимания процессов метаболизма головного мозга в норме и при различных заболеваниях.

INRODUCTION

INRODUCTION: Proton magnetic resonance spectroscopy (MR spectroscopy, MRS) allows determining tissue metabolite concentrations in the region of interest. Clinically used MR spectroscopy pulse sequences solve two main problems: voxel localization and water signal suppression. The spectra obtained in this way are a superposition of all signals from the region of interest, so the most reliable information can be obtained only about a small number of metabolites with the highest concentrations. The remaining metabolites have low concentrations and/or have complex peak splitting, and are overlapped by more intense signals. Spectral editing is a method for identifying these metabolites in clinically available systems (<3T). This review briefly presents spectral editing methods, discusses post-processing methods for such spectra, and discusses the main results of studies using spectral editing. Spectral editing methods have shown that the concentrations of γ-aminobutyric acid, lactate, aspartate, N-acetylaspartate and N-acetylaspartylglutamate, 2-hydroxyglutarate, glutathione, and glutamate change in various diseases. The methods and research results considered in this review will allow clinicians to suggest and test new approaches to diagnosing diseases of the central nervous system. 

OBJECTIVE

OBJECTIVE: To describe methods for editing the 1H MRS spectrum in human studies and to present some of the main results of the application of these measurements.

CONCLUSION

CONCLUSION: Using the most effective editing methods to optimize information in human brain proton spectra is a highly complex technical process. However, the application of the editing techniques discussed in this lecture allows us to obtain unique and invaluable information on the intracellular concentrations of several key metabolites, which undoubtedly enhances our understanding of brain metabolism in health and disease.

J-связьусреднение по времени эхаредактирование разности JPRESS с постоянным временемквантовая фильтрацияметаболитымагнитно-резонансная спектроскопия (МРС)J-couplingecho time averagingJ-difference editingconstant-time PRESSquantum filteringmetabolitesmagnetic resonance spectroscopy (MRS)работа проведена в рамках выполнения программы Департамента здравоохранения города Москвы по теме «Актуальные инновационные технологии диагностики значимых последствий тяжёлых травм у детей на этапе ранней мультидисциплинарной диагностики»this work was carried out as part of the Moscow Department of Health program on the theme «Current innovative technologies for diagnosing significant consequences of severe injuries in children at the stage of early multidisciplinary diagnosis».ReferencesVan der Graaf M. In vivo magnetic resonance spectroscopy: basic methodology and clinical applications // Eur. Biophys. J. 2010. Vol. 39, No. 4. P. 527–540. doi: 10.1007/s00249-009-0517-y.Van der Graaf M. In vivo magnetic resonance spectroscopy: basic methodology and clinical applications // Eur. Biophys. J. 2010. Vol. 39, No. 4. P. 527–540. doi: 10.1007/s00249-009-0517-y.Veeraiah P., Jansen J.F.A. Multinuclear Magnetic Resonance Spectroscopy at Ultra-High-Field: Assessing Human Cerebral Metabolism in Healthy and Diseased States // Metabolites. 2023. Vol. 13, No. 4. P. 577. doi: 10.3390/metabo13040577.Veeraiah P., Jansen J.F.A. Multinuclear Magnetic Resonance Spectroscopy at Ultra-High-Field: Assessing Human Cerebral Metabolism in Healthy and Diseased States // Metabolites. 2023. Vol. 13, No. 4. P. 577. doi: 10.3390/metabo13040577.Yahya A., Mädler B., Fallone B.G. Exploiting the chemical shift displacement effect in the detection of glutamate and glutamine (Glx) with PRESS // J. Magn. Reson. 2008. Vol. 191, No. 1. P. 120–127. doi: 10.1016/j.jmr.2007.12.007.Yahya A., Mädler B., Fallone B.G. Exploiting the chemical shift displacement effect in the detection of glutamate and glutamine (Glx) with PRESS // J. Magn. Reson. 2008. Vol. 191, No. 1. P. 120–127. doi: 10.1016/j.jmr.2007.12.007.Choi C., Ganji S.K., DeBerardinis R.J. et al. 2-hydroxyglutarate detection by magnetic resonance spectroscopy in IDH-mutated patients with gliomas // Nat. Med. 2012. Vol. 18, No. 4. Р. 624–629. doi: 10.1038/nm.2682.Choi C., Ganji S.K., DeBerardinis R.J. et al. 2-hydroxyglutarate detection by magnetic resonance spectroscopy in IDH-mutated patients with gliomas // Nat. Med. 2012. Vol. 18, No. 4. Р. 624–629. doi: 10.1038/nm.2682.Hurd R., Sailasuta N., Srinivasan R. et al. Measurement of Brain Glutamate Using TE-Averaged PRESS at 3T // Magn. Reson. Med. 2004. Vol. 51, No. 3. P. 435440. doi: 10.1002/mrm.20007.Hurd R., Sailasuta N., Srinivasan R. et al. Measurement of Brain Glutamate Using TE-Averaged PRESS at 3T // Magn. Reson. Med. 2004. Vol. 51, No. 3. P. 435440. doi: 10.1002/mrm.20007.Hancu I. Optimized Glutamate Detection at 3T // J. Magn. Reson. Imaging. 2009. Vol. 30, No. 5. P. 1155–1162. doi: 10.1002/jmri.21936.Hancu I. Optimized Glutamate Detection at 3T // J. Magn. Reson. Imaging. 2009. Vol. 30, No. 5. P. 1155–1162. doi: 10.1002/jmri.21936.Choi I.Y., Andronesi O.C., Barker P. et al. Spectral editing in 1H magnetic resonance spectroscopy: Experts’ consensus recommendations // NMR in Biomedicine. 2021. Vol. 34, No. 5. e4411. doi: 10.1002/nbm.4411.Choi I.Y., Andronesi O.C., Barker P. et al. Spectral editing in 1H magnetic resonance spectroscopy: Experts’ consensus recommendations // NMR in Biomedicine. 2021. Vol. 34, No. 5. e4411. doi: 10.1002/nbm.4411.McLean M.A., Busza A.L., Wald L.L. et al. In vivo GABA+ measurement at 1.5T using a PRESS‐localized double quantum filter // Magn. Reson. Med. 2002. Vol. 48. P. 233–241. doi: 10.1002/mrm.10208.McLean M.A., Busza A.L., Wald L.L. et al. In vivo GABA+ measurement at 1.5T using a PRESS‐localized double quantum filter // Magn. Reson. Med. 2002. Vol. 48. P. 233–241. doi: 10.1002/mrm.10208.Edden R.A.E., Puts N.A.J., Harris A.D., Barker P.B., Evans C.J. Gannet: A batch-processing tool for the quantitative analysis of gamma-aminobutyric acid-edited MR spectroscopy spectra // Journal of Magnetic Resonance Imaging. 2014. Vol. 40, No. 6. P. 1445–1452. doi: 10.1002/jmri.24478.Edden R.A.E., Puts N.A.J., Harris A.D., Barker P.B., Evans C.J. Gannet: A batch-processing tool for the quantitative analysis of gamma-aminobutyric acid-edited MR spectroscopy spectra // Journal of Magnetic Resonance Imaging. 2014. Vol. 40, No. 6. P. 1445–1452. doi: 10.1002/jmri.24478.Provencher S.W. Estimation of metabolite concentrations from localized in vivo proton NMR spectra // Magnetic Resonance in Medicine. 1993. Vol. 30, No. 6. P. 672–679. doi: 10.1002/mrm.1910300604.Provencher S.W. Estimation of metabolite concentrations from localized in vivo proton NMR spectra // Magnetic Resonance in Medicine. 1993. Vol. 30, No. 6. P. 672–679. doi: 10.1002/mrm.1910300604.Hui S.C.N., Saleh M.G., Zöllner H.J. et al. MRSCloud A cloud‐based MRS tool for basis set simulation // Magnetic Resonance in Med. 2022. Vol. 88, No. 5. P. 1994–2004. doi: 10.1002/mrm.29370.Hui S.C.N., Saleh M.G., Zöllner H.J. et al. MRSCloud A cloud‐based MRS tool for basis set simulation // Magnetic Resonance in Med. 2022. Vol. 88, No. 5. P. 1994–2004. doi: 10.1002/mrm.29370.Near J., Harris A.D., Juchem C. et al. Preprocessing, analysis and quantification in single‐voxel magnetic resonance spectroscopy: experts’ consensus recommendations // NMR in Biomedicine. 2021. Vol. 34, No. 5. e4257. doi: 10.1002/nbm.4257.Near J., Harris A.D., Juchem C. et al. Preprocessing, analysis and quantification in single‐voxel magnetic resonance spectroscopy: experts’ consensus recommendations // NMR in Biomedicine. 2021. Vol. 34, No. 5. e4257. doi: 10.1002/nbm.4257.O’Gorman R.L., Michels L., Edden R.A., Murdoch J.B., Martin E. In vivo detection of GABA and glutamate with MEGA-PRESS: reproducibility and gender effects // J. Magn. Reson. Imaging. 2011. Vol. 33, No. 5. P. 1262–1267. doi: 10.1002/jmri.22520.O’Gorman R.L., Michels L., Edden R.A., Murdoch J.B., Martin E. In vivo detection of GABA and glutamate with MEGA-PRESS: reproducibility and gender effects // J. Magn. Reson. Imaging. 2011. Vol. 33, No. 5. P. 1262–1267. doi: 10.1002/jmri.22520.Craven A.R., Bhattacharyya P.K., Clarke W.T. et al. Comparison of seven modelling algorithms for g‐aminobutyric acid-edited proton magnetic resonance spectroscopy // NMR in Biomedicine. 2022. Vol. 35, No. 7. e4702. doi: 10.1002/nbm.4702.Craven A.R., Bhattacharyya P.K., Clarke W.T. et al. Comparison of seven modelling algorithms for g‐aminobutyric acid-edited proton magnetic resonance spectroscopy // NMR in Biomedicine. 2022. Vol. 35, No. 7. e4702. doi: 10.1002/nbm.4702.Govindaraju V., Young K., Maudsley A.A. Proton NMR chemical shifts and coupling constants for brain metabolites // NMR in Biomedicine. 2000. Vol. 13, No. 3. P. 129–153. doi: 10.1002/1099–1492(200005)13:3<129::aid-nbm619>3.0.co;2-v.Govindaraju V., Young K., Maudsley A.A. Proton NMR chemical shifts and coupling constants for brain metabolites // NMR in Biomedicine. 2000. Vol. 13, No. 3. P. 129–153. doi: 10.1002/1099–1492(200005)13:3<129::aid-nbm619>3.0.co;2-v.Klunk W.E., Xu C., Panchalingram K., Mcclure R.J., Pettegrew J.W. Quantitative 1H and 31P MRS of PCA extracts of postmortem Alzheimer’s disease brain // Neurobiology of Aging. 1996. Vol. 17, No. 3. P. 349–357. doi: 10.1016/0197-4580(96)00035-8.Klunk W.E., Xu C., Panchalingram K., Mcclure R.J., Pettegrew J.W. Quantitative 1H and 31P MRS of PCA extracts of postmortem Alzheimer’s disease brain // Neurobiology of Aging. 1996. Vol. 17, No. 3. P. 349–357. doi: 10.1016/0197-4580(96)00035-8.Menshchikov P.E., Semenova N.A., Akhadov T.A., Bozhko O.V., Varfolomeev S.D. An Increase in Cerebral g-Amino Butyric Acid Concentration in Children with Mild Traumatic Brain Injury in the Acute Phase: 1H MRS Study // Biophysics (Rus.). 2017. Vol. 62. P. 1009–1018.Menshchikov P.E., Semenova N.A., Akhadov T.A., Bozhko O.V., Varfolomeev S.D. An Increase in Cerebral g-Amino Butyric Acid Concentration in Children with Mild Traumatic Brain Injury in the Acute Phase: 1H MRS Study // Biophysics (Rus.). 2017. Vol. 62. P. 1009–1018.Nadler J.V., Cooper J.R. N‐acetyl‐L‐aspartic acid content of human neural tumours and bovine peripheral nervous tissues // Journal of Neurochemistry. 1972. Vol. 19. P. 313–319. doi: 10.1111/j.1471-4159.1972.tb01341.x.Nadler J.V., Cooper J.R. N‐acetyl‐L‐aspartic acid content of human neural tumours and bovine peripheral nervous tissues // Journal of Neurochemistry. 1972. Vol. 19. P. 313–319. doi: 10.1111/j.1471-4159.1972.tb01341.x.Menshchikov P., Ivantsova A., Manzhurtsev A. et al. Separate N‐acetyl aspartyl glutamate, N‐acetyl aspartate, aspartate, and glutamate quantification after pediatric mild traumatic brain injury in the acute phase // Magn. Reson. Med. 2020. Vol. 84, No. 6. P. 2918–2931. doi: 10.1002/mrm.28332.Menshchikov P., Ivantsova A., Manzhurtsev A. et al. Separate N‐acetyl aspartyl glutamate, N‐acetyl aspartate, aspartate, and glutamate quantification after pediatric mild traumatic brain injury in the acute phase // Magn. Reson. Med. 2020. Vol. 84, No. 6. P. 2918–2931. doi: 10.1002/mrm.28332.Landim R.C.G., Edden R.A.E., Foerster B. et al. Investigation of NAA and NAAG dynamics underlying visual stimulation using MEGA-PRESS in a functional MRS experiment // Magn. Reson. Imaging. 2016. Vol. 34, No. 3. P. 239–245. doi: 10.1016/j.mri.2015.10.038.Landim R.C.G., Edden R.A.E., Foerster B. et al. Investigation of NAA and NAAG dynamics underlying visual stimulation using MEGA-PRESS in a functional MRS experiment // Magn. Reson. Imaging. 2016. Vol. 34, No. 3. P. 239–245. doi: 10.1016/j.mri.2015.10.038.Rowland L.M., Kontson K., West J. et al. In vivo measurements of glutamate, GABA, and NAAG in schizophrenia // Schizophr Bull. 2013. Vol. 39, No. 5. P. 10961104. doi: 10.1093/schbul/sbs092.Rowland L.M., Kontson K., West J. et al. In vivo measurements of glutamate, GABA, and NAAG in schizophrenia // Schizophr Bull. 2013. Vol. 39, No. 5. P. 10961104. doi: 10.1093/schbul/sbs092.Pouwels P.J.W., Frahm J. Differential distribution of NAA and NAAG in human brain as determined by quantitative localized proton MRS // NMR Biomed. 1997. Vol. 10, No. 2. P. 73–78.Pouwels P.J.W., Frahm J. Differential distribution of NAA and NAAG in human brain as determined by quantitative localized proton MRS // NMR Biomed. 1997. Vol. 10, No. 2. P. 73–78.McKenna M.C. The glutamate-glutamine cycle is not stoichiometric: Fates of glutamate in brain // Journal of Neuroscience Research. 2007. Р. 3347–3358.McKenna M.C. The glutamate-glutamine cycle is not stoichiometric: Fates of glutamate in brain // Journal of Neuroscience Research. 2007. Р. 3347–3358.Yakovlev A., Manzhurtsev A., Menshchikov P. et al. Functional Magnetic Resonance Spectroscopy Study of Total Glutamate and Glutamine in the Human Visual Cortex Activated by a Short Stimulus // Biophysics. 2022. Vol. 67, No. 2. P. 265–273. doi: 10.1134/S0006350922020245.Yakovlev A., Manzhurtsev A., Menshchikov P. et al. Functional Magnetic Resonance Spectroscopy Study of Total Glutamate and Glutamine in the Human Visual Cortex Activated by a Short Stimulus // Biophysics. 2022. Vol. 67, No. 2. P. 265–273. doi: 10.1134/S0006350922020245.Han J., Ma L. Study of the features of proton MR spectroscopy ((1)H-MRS) on amyotrophic lateral sclerosis // J. Magn. Reson. Imaging. 2010. Vol. 31, No. 2. P. 305–308. doi: 10.1002/jmri.22053.Han J., Ma L. Study of the features of proton MR spectroscopy ((1)H-MRS) on amyotrophic lateral sclerosis // J. Magn. Reson. Imaging. 2010. Vol. 31, No. 2. P. 305–308. doi: 10.1002/jmri.22053.Marsman A., Van Den Heuvel M.P., Klomp D.W.J. et al. Glutamate in Schizophrenia: A Focused Review and Meta-Analysis of 1H-MRS Studies // Schizophrenia Bulletin. 2013. Vol. 39, No. 1. P. 120–129. doi: 10.1093/schbul/sbr069.Marsman A., Van Den Heuvel M.P., Klomp D.W.J. et al. Glutamate in Schizophrenia: A Focused Review and Meta-Analysis of 1H-MRS Studies // Schizophrenia Bulletin. 2013. Vol. 39, No. 1. P. 120–129. doi: 10.1093/schbul/sbr069.Ramadan S., Lin A., Stanwell P. Glutamate and glutamine: A review of in vivo MRS in the human brain // NMR in Biomedicine. 2013. Vol. 26, No. 12. P. 16301646. doi: 10.1002/nbm.3045.Ramadan S., Lin A., Stanwell P. Glutamate and glutamine: A review of in vivo MRS in the human brain // NMR in Biomedicine. 2013. Vol. 26, No. 12. P. 16301646. doi: 10.1002/nbm.3045.Gonenc A., Govind V., Sheriff S., Maudsley A.A. Comparison of spectral fitting methods for overlapping J-coupled metabolite resonances // Magn. Reson. Med. 2010. Vol. 64, No. 3. P. 623–628. doi: 10.1002/mrm.22540.Gonenc A., Govind V., Sheriff S., Maudsley A.A. Comparison of spectral fitting methods for overlapping J-coupled metabolite resonances // Magn. Reson. Med. 2010. Vol. 64, No. 3. P. 623–628. doi: 10.1002/mrm.22540.Wijtenburg S.A., Knight-Scott J. Very short echo time improves the precision of glutamate detection at 3T in 1H magnetic resonance spectroscopy // Journal of Magnetic Resonance Imaging. 2011. Vol. 34, No. 3. P. 645–652. doi: 10.1002/jmri.22638.Wijtenburg S.A., Knight-Scott J. Very short echo time improves the precision of glutamate detection at 3T in 1H magnetic resonance spectroscopy // Journal of Magnetic Resonance Imaging. 2011. Vol. 34, No. 3. P. 645–652. doi: 10.1002/jmri.22638.Snyder J., Wilman A. Field strength dependence of PRESS timings for simultaneous detection of glutamate and glutamine from 1.5 to 7 T // Journal of Magnetic Resonance. 2010. Vol. 203, No. 1. P. 66–72. doi: 10.1016/j.jmr.2009.12.002.Snyder J., Wilman A. Field strength dependence of PRESS timings for simultaneous detection of glutamate and glutamine from 1.5 to 7 T // Journal of Magnetic Resonance. 2010. Vol. 203, No. 1. P. 66–72. doi: 10.1016/j.jmr.2009.12.002.Schubert F., Gallinat J., Seifert F., Rinneberg H. Glutamate concentrations in human brain using single voxel proton magnetic resonance spectroscopy at 3 Tesla // NeuroImage. 2004. Vol. 21, No. 4. P. 1762–1771. doi: 10.1016/j.neuroimage.2003.11.014.Schubert F., Gallinat J., Seifert F., Rinneberg H. Glutamate concentrations in human brain using single voxel proton magnetic resonance spectroscopy at 3 Tesla // NeuroImage. 2004. Vol. 21, No. 4. P. 1762–1771. doi: 10.1016/j.neuroimage.2003.11.014.Ferguson B.R., Gao W-J. PV Interneurons: Critical Regulators of E/I Balance for Prefrontal Cortex-Dependent Behavior and Psychiatric Disorders // Front Neural Circuits. 2018. Vol. 12. P. 37. doi: 10.3389/fncir.2018.00037.Ferguson B.R., Gao W-J. PV Interneurons: Critical Regulators of E/I Balance for Prefrontal Cortex-Dependent Behavior and Psychiatric Disorders // Front Neural Circuits. 2018. Vol. 12. P. 37. doi: 10.3389/fncir.2018.00037.Donahue M.J., Rane S., Hussey E. et al. g-Aminobutyric Acid (GABA) Concentration Inversely Correlates with Basal Perfusion in Human Occipital Lobe // J. Cereb. Blood Flow Metab. 2014. Vol. 34. P. 532–541.Donahue M.J., Rane S., Hussey E. et al. g-Aminobutyric Acid (GABA) Concentration Inversely Correlates with Basal Perfusion in Human Occipital Lobe // J. Cereb. Blood Flow Metab. 2014. Vol. 34. P. 532–541.Yakovlev A., Gritskova A., Manzhurtsev A. et al. Dynamics of g-aminobutyric acid concentration in the human brain in response to short visual stimulation // MAGMA 2024. Vol. 37, No. 1. P. 39–51. doi: 10.1007/s10334-023-01118-7.Yakovlev A., Gritskova A., Manzhurtsev A. et al. Dynamics of g-aminobutyric acid concentration in the human brain in response to short visual stimulation // MAGMA 2024. Vol. 37, No. 1. P. 39–51. doi: 10.1007/s10334-023-01118-7.Gao F., Edden R.A.E., Li M. et al. Edited magnetic resonance spectroscopy detects an age-related decline in brain GABA levels // Neuroimage. 2013. Vol. 78. P. 7582. doi: 10.1016/j.neuroimage.2013.04.012.Gao F., Edden R.A.E., Li M. et al. Edited magnetic resonance spectroscopy detects an age-related decline in brain GABA levels // Neuroimage. 2013. Vol. 78. P. 7582. doi: 10.1016/j.neuroimage.2013.04.012.Bollmann S., Ghisleni C., Poil S-S. et al. Developmental changes in gamma-aminobutyric acid levels in attention-deficit/hyperactivity disorder // Transl. Psychiatry 2015. Vol. 5, No. 6. e589. doi: 10.1038/tp.2015.79.Bollmann S., Ghisleni C., Poil S-S. et al. Developmental changes in gamma-aminobutyric acid levels in attention-deficit/hyperactivity disorder // Transl. Psychiatry 2015. Vol. 5, No. 6. e589. doi: 10.1038/tp.2015.79.Simister R.J., McLean M.A., Barker G.J., Duncan J.S. Proton magnetic resonance spectroscopy of malformations of cortical development causing epilepsy // Epilepsy Res. 2007. Vol. 74. P. 107–115. doi: 10.1016/j.eplepsyres.2007.02.002.Simister R.J., McLean M.A., Barker G.J., Duncan J.S. Proton magnetic resonance spectroscopy of malformations of cortical development causing epilepsy // Epilepsy Res. 2007. Vol. 74. P. 107–115. doi: 10.1016/j.eplepsyres.2007.02.002.Foerster B.R., Petrou M., Edden R.A.E. et al. Reduced insular g-aminobutyric acid in fibromyalgia // Arthritis Rheum. 2012. Vol. 64, No. 2. P. 579–583. doi: 10.1002/art.33339.Foerster B.R., Petrou M., Edden R.A.E. et al. Reduced insular g-aminobutyric acid in fibromyalgia // Arthritis Rheum. 2012. Vol. 64, No. 2. P. 579–583. doi: 10.1002/art.33339.Mullins P.G., McGonigle D.J., O’Gorman R.L. et al. Current practice in the use of MEGA-PRESS spectroscopy for the detection of GABA // NeuroImage. 2014. Vol. 86. P. 43–52. doi: 10.1016/j.neuroimage.2012.12.004.Mullins P.G., McGonigle D.J., O’Gorman R.L. et al. Current practice in the use of MEGA-PRESS spectroscopy for the detection of GABA // NeuroImage. 2014. Vol. 86. P. 43–52. doi: 10.1016/j.neuroimage.2012.12.004.Yakovlev A., Manzhurtsev A., Menshchikov P. et al. The Effect of Visual Stimulation on GABA and Macromolecule Levels in the Human Brain in vivo // Biophysics (Russian Federation). 2020. Vol. 65. P. 51–57.Yakovlev A., Manzhurtsev A., Menshchikov P. et al. The Effect of Visual Stimulation on GABA and Macromolecule Levels in the Human Brain in vivo // Biophysics (Russian Federation). 2020. Vol. 65. P. 51–57.Mangia S., Tkáč I., Gruetter R. et al. Sustained neuronal activation raises oxidative metabolism to a new steady-state level: Evidence from[1] H NMR spectroscopy in the human visual cortex // Journal of Cerebral Blood Flow and Metabolism. 2007. Vol. 27, No. 5. P. 1055–1063. doi: 10.1038/sj.jcbfm.9600401.Mangia S., Tkáč I., Gruetter R. et al. Sustained neuronal activation raises oxidative metabolism to a new steady-state level: Evidence from[1] H NMR spectroscopy in the human visual cortex // Journal of Cerebral Blood Flow and Metabolism. 2007. Vol. 27, No. 5. P. 1055–1063. doi: 10.1038/sj.jcbfm.9600401.Parsons D.W., Jones S., Zhang X. et al. An Integrated Genomic Analysis of Human Glioblastoma Multiforme // Science. 2008. Vol. 321. P. 1807–1812. doi: 10.1126/science.1164382.Parsons D.W., Jones S., Zhang X. et al. An Integrated Genomic Analysis of Human Glioblastoma Multiforme // Science. 2008. Vol. 321. P. 1807–1812. doi: 10.1126/science.1164382.Pope W.B., Prins R.M., Albert Th.M. et al. Non-invasive detection of 2-hydroxyglutarate and other metabolites in IDH1 mutant glioma patients using magnetic resonance spectroscopy // J. Neuroonco l. 2012. Vol. 107, No. 1. P. 197–205. doi: 10.1007/s11060-011-0737-8.Pope W.B., Prins R.M., Albert Th.M. et al. Non-invasive detection of 2-hydroxyglutarate and other metabolites in IDH1 mutant glioma patients using magnetic resonance spectroscopy // J. Neuroonco l. 2012. Vol. 107, No. 1. P. 197–205. doi: 10.1007/s11060-011-0737-8.Andronesi O.C., Kim G.S., Gerstner E. et al. Detection of 2-Hydroxyglutarate in IDH-Mutated Glioma Patients by In Vivo Spectral-Editing and 2D Correlation Magnetic Resonance Spectroscopy // Sci. Transl. Med. 2012. doi: 10.1126/scitranslmed.3002693.Andronesi O.C., Kim G.S., Gerstner E. et al. Detection of 2-Hydroxyglutarate in IDH-Mutated Glioma Patients by In Vivo Spectral-Editing and 2D Correlation Magnetic Resonance Spectroscopy // Sci. Transl. Med. 2012. doi: 10.1126/scitranslmed.3002693.Dwivedi D., Megha K., Mishra R., Mandal P.K. Glutathione in Brain: Overview of Its Conformations, Functions, Biochemical Characteristics, Quantitation and Potential Therapeutic Role in Brain Disorders // Neurochem. Res. 2020. Vol. 45, No. 7. P. 1461–1480. doi: 10.1007/s11064-020-03030-1.Dwivedi D., Megha K., Mishra R., Mandal P.K. Glutathione in Brain: Overview of Its Conformations, Functions, Biochemical Characteristics, Quantitation and Potential Therapeutic Role in Brain Disorders // Neurochem. Res. 2020. Vol. 45, No. 7. P. 1461–1480. doi: 10.1007/s11064-020-03030-1.Marí M., De Gregorio E., De Dios C. et al. Mitochondrial Glutathione: Recent Insights and Role in Disease // Antioxidants. 2020. Vol. 9, No. 10. P. 909. doi: 10.3390/antiox9100909.Marí M., De Gregorio E., De Dios C. et al. Mitochondrial Glutathione: Recent Insights and Role in Disease // Antioxidants. 2020. Vol. 9, No. 10. P. 909. doi: 10.3390/antiox9100909.Emir U.E., Raatz S., McPherson S. et al. Noninvasive quantification of ascorbate and glutathione concentration in the elderly human brain // NMR Biomed. 2011. Vol. 2, No. 7. P. 888–894. doi: 10.1002/nbm.1646.Emir U.E., Raatz S., McPherson S. et al. Noninvasive quantification of ascorbate and glutathione concentration in the elderly human brain // NMR Biomed. 2011. Vol. 2, No. 7. P. 888–894. doi: 10.1002/nbm.1646.Bell C.J.M., Mehta M., Mirza L., Young A.H., Beck K. Glutathione alterations in depression: a meta-analysis and systematic review of proton magnetic resonance spectroscopy studies // Psychopharmacology. 2025. Vol. 242, No. 4. P. 717–724. doi: 10.1007/s00213-024-06735-1.Bell C.J.M., Mehta M., Mirza L., Young A.H., Beck K. Glutathione alterations in depression: a meta-analysis and systematic review of proton magnetic resonance spectroscopy studies // Psychopharmacology. 2025. Vol. 242, No. 4. P. 717–724. doi: 10.1007/s00213-024-06735-1.Kaiser L.G., Marjańska M., Matson G.B. et al. (1)H MRS detection of glycine residue of reduced glutathione in vivo // J. Magn. Reson. 2010. Vol. 202, No. 2. P. 259–266. doi: 10.1016/j.jmr.2009.11.013.Kaiser L.G., Marjańska M., Matson G.B. et al. (1)H MRS detection of glycine residue of reduced glutathione in vivo // J. Magn. Reson. 2010. Vol. 202, No. 2. P. 259–266. doi: 10.1016/j.jmr.2009.11.013.Chan K.L., Puts N.A.J., Snoussi K. et al. Echo time optimization for J‐difference editing of glutathione at 3T // Magnetic Resonance in Med. 2017. Vol. 77, No. 2. P. 498–504. doi: 10.1002/mrm.26122.Chan K.L., Puts N.A.J., Snoussi K. et al. Echo time optimization for J‐difference editing of glutathione at 3T // Magnetic Resonance in Med. 2017. Vol. 77, No. 2. P. 498–504. doi: 10.1002/mrm.26122.

The authors declare that there are no conflicts of interest present.