Pre-clinical study of paramagnetic contrast enhancement with Mn(II)-dimercaptosuccinate complex in magnetic resonance imaging of primary tumor and metastatic spread of breast cancer

Aim of the study. We have studied the possibility of imaging of breast cancer in dogs by MRI with paramagnetic contrast enhancement using newly proposed agent Mn-dimercaptosuccinate (Mn-DMSA₂, ^TMSuccimang), in dogs. Material and methods. In seven animals (veterinary patients) with breast cancer the MRI study was carried out, when sleeping (infusion of propofol), with contrast enhancement using Mn-DMSA₂, as 0,5M solution, 0,1 mM/Kg of BW. The T1-w MRI scans were acquired before, and in 12–17 min after injection of Mn-DMSA₂, TR=450–600 ms, TE=12–15 ms, matrix 256×256, field of view 250 mm, slice 2,5 mm thick. All studies were carried out using Toshiba Titan Vantage (1,5 Т, five animals) and Siemens Magnetom Open (0,2 Т, in two). The uptake of Mn-DMSA₂ to tumor was quantified using Index of Enhancement, as ratio of intensity per voxel of the post-contrast image to the intensity of the pre-contrast initial one. Results. In all cases the uptake of Mn-DMSA₂ was seen in the areas of both primary tumor and of metastases to lymph nodes and to the brain. Index of enhancement was: over primary tumor (n=7) — 1,52±0,19 (1,35; 1,83), over metastases to lymph nodes (n=12) 1,37±0,14 (1,12; 1,64), over peripheral zone of brain metastases (n=11) — 1,48±0,15 (1,29; 2,37), and over central zone of brain metastases (n=11) — 2,76±0,12 (1,48; 3,21). No any case demonstrated any complication or concomitant effect. Conclusion. Mn-DMSA₂ is an perspective non-gadolinium paramagnetic contrast agent useful for diagnosis of both primary tumor and of metastases in breast cancer.

MRI, paramagnetic contrast enhancement, breast cancer, Mn-dimercaptosuccinate

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